Advances in Flavivirus research applications: new techniques using the FastPrep-24 5GTM sample preparation system
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چکیده
The FastPrep-24 5G (Fig. 1) is a high-speed, benchtop reciprocating instrument that is intended for the optimal lysis of challenging and routine sample types. Its use of bead-beating technology makes it suitable in all applications that require grinding, lysing or homogenization of various solid sample materials. Mechanical sample lysis offers rapid sample preparation for the isolation of DNA, RNA, proteins, metabolites and other small molecules while eliminating the need for chemicals, enzymes and detergents, which minimizes the introduction of potential inhibitors to downstream processes. Sample types include but are not limited to the following: all types of human, animal and plant tissues, including cultured cells; bacterial, yeast and fungal cells, including spores and oocytes; and environmental and metagenomic samples, including soil and fecal samples. The FastPrep-24 5G instrument uses a unique, optimized motion to disrupt cells through the multidirectional, simultaneous beating of specialized Lysing Matrix beads on the sample material. Developed for difficult and resistant samples, the FastPrep-24 5G instrument thoroughly and quickly lyses any tissues and cells and thus allows easy and reproducible isolation of stable RNA, active proteins and full-length genomic DNA. The FastPrep-24 5G is the only available homogenizer with nine optional adaptors, which allows for unique versatility in sample number, with tube sizes of 2, 4.5, 15 and 50 ml available for processing samples under ambient or cryogenic temperature conditions. Samples and buffers are added to a Lysing Matrix tube containing specialized Lysing Matrix beads. A wide range of Lysing Matrix tubes are available and contain beads of different materials, sizes and shapes that have been optimized by sample type and guarantee thorough homogenization every time.
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تاریخ انتشار 2016